Detection of Mycobacterium avium, M. intracellulare and M. kansasii in Sputum by Two-Step PCR
نویسندگان
چکیده
منابع مشابه
Identification & differentiation of Mycobacterium avium & M. intracellulare by PCR- RFLP assay using the groES gene.
BACKGROUND & OBJECTIVE We report a new polymerase chain reaction (PCR) - restriction fragment length polymorphism (RFLP) assay using mycobacterial groES as a target to identify Mycobacterium avium and M. intracellulare in clinical samples. METHODS The assay was standardized using M. avium and M. intracellulare standard strains obtained from ATCC and was tested with 45 M. avium-M. intracellula...
متن کاملDetection of Mycobacterium avium & M. tuberculosis from human sputum cultures by PCR-RFLP analysis of hsp65 gene & pncA PCR.
BACKGROUND & OBJECTIVE Identification of mycobacteria by conventional methods is slow, labour intensive and may at times fail to produce precise results. Molecular techniques developed in the recent past, overcome these disadvantages facilitating rapid identification of most species. We undertook this study to characterize mycobacteria isolated from sputa of human patients suspected to have tub...
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The cross-reactivity of mycobacterial antigens in immune-based diagnostic assays has been a major concern and a criticism of the current tests that are used for the detection of paratuberculosis. In the present study, Mycobacterium avium subsp. paratuberculosis recombinant proteins were evaluated for antigenic specificity compared to a whole-cell sonicate preparation (MPS). Measures of cell-med...
متن کاملIdentification of cytoplasmic membrane protein antigens of Mycobacterium avium, M. intracellulare, and M. scrofulaceum.
The cytoplasmic membrane isolated from representative strains of the Mycobacterium avium, M. intracellulare, and M. scrofulaceum (MAIS) group contained approximately 20 proteins, as identified by SDS - polyacrylamide gel electrophoresis. One membrane protein predominated, comprising up to 50% of the total membrane protein. This major cytoplasmic membrane protein (MCMP) had a molecular weight of...
متن کاملRestriction endonuclease analysis of members of the Mycobacterium avium-M. intracellulare-M. scrofulaceum serocomplex.
Organisms belonging to the Mycobacterium avium-M. intracellulare-M. scrofulaceum (MAIS) serocomplex were subjected to restriction endonuclease analysis (REA) with the enzymes BstEII, PvuII, and BclI. Substantial genetic heterogeneity was observed between members of an authenticated collection of the 31 serotypes. Serotypes 2 and 3 were indistinguishable, however, as were serotypes 5 and 10. No ...
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ژورنال
عنوان ژورنال: Journal of the Japanese Association for Infectious Diseases
سال: 1994
ISSN: 1884-569X,0387-5911
DOI: 10.11150/kansenshogakuzasshi1970.68.42